Journal: Microbiome

Article Title: Antimicrobial peptides modulate lung injury by altering the intestinal microbiota

doi: 10.1186/s40168-023-01673-0

Figure Lengend Snippet: Oxygen exposure reduces intestinal antimicrobial peptide expression. A Neonatal C57BL/6 J mice were exposed to normoxia or hyperoxia from the 3 rd -14. th day of life ( n = 4 litters with 5–7 neonatal mice/litter per exposure group). FiO , fraction of inspired oxygen. SPF, specific-pathogen-free. B Representative photomicrographs of the distal lung sections of 14-day-old mice. C Hyperoxia exposure is associated with alterations in lung morphology and function. Data are shown as mean ± SEM, with significance testing by a two-tailed t -test. D Volcano plot of ileal gene expression array showing gene expression altered by hyperoxia exposure. E Heatmap showing genes regulated by hyperoxia exposure. F Principal components analysis showing differential clustering of normoxia and hyperoxia exposed ileal genes. PC, principal component. G Ileal antimicrobial peptide expression is decreased in hyperoxia-exposure mice. H Community diversity of the adherent and luminal ileal bacterial microbiome is not significantly altered by hyperoxia exposure. I The relative abundance of an operational taxonomic unit (OTU 002) that aligns to the genus Staphylococcus increases after hyperoxia exposure, as do OTUs aligning to Corynebacterium (OTU 124) and Romboutsia (OTU 013). Data are shown as mean ± SEM, with significance testing by a two-tailed t -test. J Principal coordinates analysis of Bray–Curtis dissimilarity shows global alterations in community composition in hyperoxia-exposed mice. Significance testing by permutational ANOVA (PERMANOVA), with equivocal dispersion confirmed by permutational multivariate analysis of dispersion (PERMDISP). PC, principal component. K Loading plot of principal components analysis of Hellinger transformed Euclidian distances showing the contribution of specific genera to the global community composition. Schematic in ( A ) was generated using BioRender. See also Figures S , S and S

Article Snippet: Intestinal organoids were generated and incubated in a hyperoxia chamber (StemCell Technologies) at 95% oxygen for 24 h. After the hyperoxia was completed, the supernatant was removed, and the organoids and Matrigel were collected in 0.5 mL cell recovery media (Corning) per well and placed on ice for 40 min.

Techniques: Expressing, Two Tailed Test, Dispersion, Transformation Assay, Generated

Journal: Microbiome

Article Title: Antimicrobial peptides modulate lung injury by altering the intestinal microbiota

doi: 10.1186/s40168-023-01673-0

Figure Lengend Snippet: Intestinal lysozyme supplementation reduces hyperoxia-induced lung injury. A Neonatal C57BL/6NCrl mice randomized to either every other day exposure to lysozyme by gastric gavage or their littermate controls were then exposed to normoxia or hyperoxia from the 3 rd -14. th day of life ( n = 4 litters with 5–7 neonatal mice/litter per exposure group). FiO 2 , fraction of inspired oxygen. PBS, phosphate-buffered saline (vehicle). SPF, specific-pathogen-free. B Representative photomicrographs of the distal lung sections of 14-day-old mice. C Lysozyme exposure ameliorates hyperoxia-induced disruptions in lung morphology and function. Data are shown as mean ± SEM, with significance testing by two-way ANOVA. D Volcano plot of ileal RNAseq showing gene expression altered by lysozyme exposure. E Heatmap showing genes regulated by lysozyme exposure. F Principal components analysis showing differential clustering of ileal genes in lysozyme-exposed mice. PC, principal component. G Ileal antimicrobial peptide expression is altered in lysozyme-exposed mice. H The community diversity of the adherent and luminal ileal bacterial microbiome is not significantly altered by lysozyme exposure. I The hyperoxia-induced increase in the relative abundance of operational taxonomic unit 014 ( Staphylococcus ) is ameliorated by lysozyme exposure. Multiple other genera are increased in lysozyme and hyperoxia-exposed mice. Data are shown as mean ± SEM, with significance testing by two-way ANOVA. J Principal coordinates analysis of Bray–Curtis dissimilarity shows global alterations in community composition in lysozyme-exposed mice. Significance testing by permutational ANOVA (PERMANOVA), with equivocal dispersion confirmed by permutational multivariate analysis of dispersion (PERMDISP). PC, principal component. K Loading plot of a principal components analysis of a Hellinger transformed Euclidian distance showing global community composition significantly altered in lysozyme-exposed mice. The schematic in ( A ) was generated using BioRender. See also Figure S

Article Snippet: Intestinal organoids were generated and incubated in a hyperoxia chamber (StemCell Technologies) at 95% oxygen for 24 h. After the hyperoxia was completed, the supernatant was removed, and the organoids and Matrigel were collected in 0.5 mL cell recovery media (Corning) per well and placed on ice for 40 min.

Techniques: Saline, Expressing, Dispersion, Transformation Assay, Generated

Journal: Microbiome

Article Title: Antimicrobial peptides modulate lung injury by altering the intestinal microbiota

doi: 10.1186/s40168-023-01673-0

Figure Lengend Snippet: Lysozyme exposure alters the lung transcriptome. A Volcano plot showing hyperoxia alters gene expression in vehicle-exposed controls. B Volcano plot showing lysozyme exposure alters gene expression in the lung. C Heatmap showing differentially expressed genes in vehicle-exposed controls. D Heatmap showing similarly expressed genes between all groups. E Heatmap showing differentially expressed genes in lysozyme-exposed mice. F Major pathways altered in mice only exposed to normoxia or hyperoxia. G Major pathways altered in lysozyme-exposed mice

Article Snippet: Intestinal organoids were generated and incubated in a hyperoxia chamber (StemCell Technologies) at 95% oxygen for 24 h. After the hyperoxia was completed, the supernatant was removed, and the organoids and Matrigel were collected in 0.5 mL cell recovery media (Corning) per well and placed on ice for 40 min.

Techniques: Expressing